Coding

Part:BBa_K4213007:Design

Designed by: Ioannis Retalis   Group: iGEM22_Thessaly   (2022-08-26)


PHT1;6 from O. sativa partially optimized, with a linker for a reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 621
    Illegal BamHI site found at 1375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1293
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

multiple mutations to make it purchase-able from IDT


Source

rice

References